HOMER1 and signalling

    81 sentences in 55 studies


  1. Saikosaponin D exerts antidepressant effect by regulating Homer1 mGluR5 and mTOR signaling in a rat model of chronic unpredictable mild stress. PMID:35610650
  2. SSD treatment reduces glutamate levels in the CA1 region and promotes the expression of the synaptic proteins PSD 95 and SYP via the regulation of the Homer1 mGluR5 and downstream mTOR signaling pathways. PMID:35610650
  3. Moreover, six candidate genes (HOMER1, PIK3CG, PIK3CA, VCAN, FABP3, and FKBP1B), functionally related to muscle development, phosphatidylinositol phosphorylation, and lipid binding, were detected around these significant SNPs. PMID:35464836
  4. Moreover, six candidate genes (HOMER1, PIK3CG, PIK3CA, VCAN, FABP3, and FKBP1B), functionally related to muscle development, phosphatidylinositol phosphorylation, and lipid binding, were detected around these significant SNPs. PMID:35464836
  5. Overexpression of Homer1 inhibits the activation of MAPK signaling, whereas Homer1 knock down increases the expression of pathway related proteins. PMID:35287697
  6. The Homer1 family of proteins at the crossroad of dopamine glutamate signaling: An emerging molecular "Lego" in the pathophysiology of psychiatric disorders. PMID:35248676
  7. Once considered only scaffolding proteins at glutamatergic postsynaptic density (PSD), Homer1 proteins are increasingly emerging as multimodal adaptors that integrate different signal transduction pathways within PSD, involved in motor and cognitive functions, with putative implications in psychiatric disorders. PMID:35248676
  8. Regulation of type I metabotropic glutamate receptor trafficking, modulation of calcium signaling, tuning of long term potentiation, organization of dendritic spines' growth, as well as meta and homeostatic plasticity control are only a few of the multiple endocellular and synaptic functions that have been linked to Homer1. PMID:35248676
  9. Homer1 is abundantly expressed at glutamatergic synapses, and is known to alter its binding to metabotropic glutamate receptor 5 (mGlu5) in response to synaptic activity. PMID:35217690
  10. Evaluation of NMD resulted in stable transcripts, and the truncated SHANK1 completely lost binding with Homer1, a linker protein that binds to the C terminus of SHANK1. PMID:34113010
  11. To independently test whether heterologous synapse formation depends on AKT signaling, we targeted PTEN, an enzyme that hydrolyzes phosphatidylinositol 3 phosphate and thereby prevents AKT kinase activation, to postsynaptic sites by fusing PTEN to Homer1. PMID:33431662
  12. To independently test whether heterologous synapse formation depends on AKT signaling, we targeted PTEN, an enzyme that hydrolyzes phosphatidylinositol 3 phosphate and thereby prevents AKT kinase activation, to postsynaptic sites by fusing PTEN to Homer1. PMID:33431662
  13. HOMER family scaffolding proteins (HOMER1 3) play critical roles in the development and progression of human disease by regulating the assembly of signal transduction complexes in response to extrinsic stimuli. PMID:33407765
  14. The data demonstrated that cyclin B2 (CCNB2), cyclin E2 (CCNE2), cyclin dependent kinase 4 (CDK4), homer scaffold protein 1 (HOMER1), heat shock protein 90 α family class A member 1 (HSP90AA1), neuroblastoma RAS viral oncogene homolog (NRAS), protein phosphatase 2 catalytic subunit α (PPP2CA), protein kinase AMP activated catalytic subunit α2 (PRKAA2) and Rac family small GTPase 1 (RAC1) may be target genes of let 7c 5p. PMID:33376529
  15. Among them, seven genes (CCNE2, CDK4, HSP90AA1, NRAS, PPP2CA, PRKAA2 and RAC1) belonged to the PI3K Akt signaling pathway and four genes (CCNB2, HOMER1, NRAS and PRKAA2) belonged to the FoxO signaling pathway. PMID:33376529
  16. The quantitative analyses of the methylation of 7 CpG sites, located on the Homer1 promoter region containing several CRE binding sites, show a significant increase in DNA methylation in the cortex of CDM mice. PMID:33128940
  17. Specifically, we investigated calcium/calmodulin dependent protein kinase II beta (CaMKIIB), homer scaffold protein 1 (HOMER1), serine/threonine protein kinase (Akt), and mechanistic target of rapamycin kinase (mTOR) following singing and non singing states in Area X. PMID:33031871
  18. Bicuculline regulated protein synthesis is dependent on Homer1 and promotes its interaction with eEF2K through mTORC1 dependent phosphorylation. PMID:32892352
  19. Together, these data underscored the involvement of ATM in a variety of neuronal vesicular trafficking processes, offering new and therapeutically useful insights into the pathogenesis of A T.Abbreviations: 3 MA: 3 methyladenine; A T: ataxia telangiectasia; ALG2: asparagine linked glycosylation 2 (alpha 1,3 mannosyltransferase); AMPK: adenosine 5' monophosphate (AMP) activated protein kinase; ATG5: autophagy related 5; ATM: ataxia telangiectasia mutated; ATP6V1A: ATPase, H+ transporting, lysosomal V1 subunit A; ATR: ataxia telangiectasia and Rad3 related; BFA1: bafilomycin A1; CC3: cleaved CASP3; CGN: cerebellar granule neuron; CLQ: chloroquine; CN: neocortical neuron; CTSB: cathepsin B; CTSD: cathepsin D; DYNLL1: the light chain1 of dynein; EIF4EBP1/4E BP1: eukaryotic translation initiation factor 4E binding protein 1; Etop: etoposide; FBS: fetal bovine serum; GAPDH: glyceraldehyde 3 phosphate dehydrogenase; HBS: HEPES buffered saline; HEPES: 4 (2 hydroxyethyl) 1 piperazineethanesulfonic acid; HOMER1: homer protein homolog 1; KU: KU 60019; LAMP1: lysosomal associated membrane protein 1; LC3B II: LC3 phosphatidylethanolamine conjugate; Lyso: lysosome; LysopH GFP: lysopHluorin GFP; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MAP2: microtubule associated protein 2; MAPK14: mitogen activated protein kinase 14; MAPK8/JNK1: mitogen activated protein kinase 8; MCOLN1/TRPML1: mucolipin 1; OSBPL1A: oxysterol binding protein like 1A; PIKK: phosphatidylinositol 3 kinase related kinase; Rapa: rapamycin; RILP: rab interacting lysosomal protein; ROS: reactive oxygen species; SEM: standard error of mean; SLC2A4/GLUT4: solute carrier family 2 (facilitated glucose transporter), member 4; TSC2/tuberin: TSC complex subunit 2; ULK1: unc 51 like kinase 1; UPS: ubiquitin proteasome system; VE: VE 822; WCL: whole cell lysate; WT: wild type. PMID:32757690
  20. Together, these data underscored the involvement of ATM in a variety of neuronal vesicular trafficking processes, offering new and therapeutically useful insights into the pathogenesis of A T.Abbreviations: 3 MA: 3 methyladenine; A T: ataxia telangiectasia; ALG2: asparagine linked glycosylation 2 (alpha 1,3 mannosyltransferase); AMPK: adenosine 5' monophosphate (AMP) activated protein kinase; ATG5: autophagy related 5; ATM: ataxia telangiectasia mutated; ATP6V1A: ATPase, H+ transporting, lysosomal V1 subunit A; ATR: ataxia telangiectasia and Rad3 related; BFA1: bafilomycin A1; CC3: cleaved CASP3; CGN: cerebellar granule neuron; CLQ: chloroquine; CN: neocortical neuron; CTSB: cathepsin B; CTSD: cathepsin D; DYNLL1: the light chain1 of dynein; EIF4EBP1/4E BP1: eukaryotic translation initiation factor 4E binding protein 1; Etop: etoposide; FBS: fetal bovine serum; GAPDH: glyceraldehyde 3 phosphate dehydrogenase; HBS: HEPES buffered saline; HEPES: 4 (2 hydroxyethyl) 1 piperazineethanesulfonic acid; HOMER1: homer protein homolog 1; KU: KU 60019; LAMP1: lysosomal associated membrane protein 1; LC3B II: LC3 phosphatidylethanolamine conjugate; Lyso: lysosome; LysopH GFP: lysopHluorin GFP; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MAP2: microtubule associated protein 2; MAPK14: mitogen activated protein kinase 14; MAPK8/JNK1: mitogen activated protein kinase 8; MCOLN1/TRPML1: mucolipin 1; OSBPL1A: oxysterol binding protein like 1A; PIKK: phosphatidylinositol 3 kinase related kinase; Rapa: rapamycin; RILP: rab interacting lysosomal protein; ROS: reactive oxygen species; SEM: standard error of mean; SLC2A4/GLUT4: solute carrier family 2 (facilitated glucose transporter), member 4; TSC2/tuberin: TSC complex subunit 2; ULK1: unc 51 like kinase 1; UPS: ubiquitin proteasome system; VE: VE 822; WCL: whole cell lysate; WT: wild type. PMID:32757690
  21. Together, these data underscored the involvement of ATM in a variety of neuronal vesicular trafficking processes, offering new and therapeutically useful insights into the pathogenesis of A T.Abbreviations: 3 MA: 3 methyladenine; A T: ataxia telangiectasia; ALG2: asparagine linked glycosylation 2 (alpha 1,3 mannosyltransferase); AMPK: adenosine 5' monophosphate (AMP) activated protein kinase; ATG5: autophagy related 5; ATM: ataxia telangiectasia mutated; ATP6V1A: ATPase, H+ transporting, lysosomal V1 subunit A; ATR: ataxia telangiectasia and Rad3 related; BFA1: bafilomycin A1; CC3: cleaved CASP3; CGN: cerebellar granule neuron; CLQ: chloroquine; CN: neocortical neuron; CTSB: cathepsin B; CTSD: cathepsin D; DYNLL1: the light chain1 of dynein; EIF4EBP1/4E BP1: eukaryotic translation initiation factor 4E binding protein 1; Etop: etoposide; FBS: fetal bovine serum; GAPDH: glyceraldehyde 3 phosphate dehydrogenase; HBS: HEPES buffered saline; HEPES: 4 (2 hydroxyethyl) 1 piperazineethanesulfonic acid; HOMER1: homer protein homolog 1; KU: KU 60019; LAMP1: lysosomal associated membrane protein 1; LC3B II: LC3 phosphatidylethanolamine conjugate; Lyso: lysosome; LysopH GFP: lysopHluorin GFP; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MAP2: microtubule associated protein 2; MAPK14: mitogen activated protein kinase 14; MAPK8/JNK1: mitogen activated protein kinase 8; MCOLN1/TRPML1: mucolipin 1; OSBPL1A: oxysterol binding protein like 1A; PIKK: phosphatidylinositol 3 kinase related kinase; Rapa: rapamycin; RILP: rab interacting lysosomal protein; ROS: reactive oxygen species; SEM: standard error of mean; SLC2A4/GLUT4: solute carrier family 2 (facilitated glucose transporter), member 4; TSC2/tuberin: TSC complex subunit 2; ULK1: unc 51 like kinase 1; UPS: ubiquitin proteasome system; VE: VE 822; WCL: whole cell lysate; WT: wild type. PMID:32757690
  22. Homer proteins act in the trafficking and/or clustering of metabotropic glutamate receptors, and polymorphisms in the HOMER1 gene have been associated with phenotypes related to glutamate signaling dysregulation. PMID:32645048
  23. Homer1 is a Potential Biomarker for Prognosis in Human Colorectal Carcinoma, Possibly in Association with G3BP1 Signaling. PMID:32425603
  24. Homer scaffolding protein 1 (Homer1) is a postsynaptic scaffold protein that regulates the structure and function of excitatory synaptic as well as its intracellular signal transduction. PMID:32425603
  25. Our findings suggest that Homer1 may play a role in malignancy of colorectal cancer mainly through the G3BP1 signaling pathway, which might be a potential indicator of poor prognosis. PMID:32425603
  26. In mammals Homer1, Homer2 and Homer3 constitute a family of scaffolding proteins with key roles in Ca2+ signaling and Ca2+ transport. PMID:32070057
  27. Transcriptional corepressor SIN3A regulates hippocampal synaptic plasticity via Homer1/mGluR5 signaling. PMID:32069266
  28. Our studies define a critical role for corepressors in modulating neural plasticity and memory consolidation and reveal that Homer1/mGluR signaling pathways may be central molecular mechanisms for memory enhancement. PMID:32069266
  29. To this end, we identified a binding site for miR 1322 via bioinformatics, quantitative reverse transcriptase polymerase chain reaction (qRT PCR), and dual luciferase reporter assays providing evidence of a direct link between circ HOMER1 and miR 1322. PMID:32037619
  30. At the molecular level, FG 4592 was found to activate HIF 1 and cAMP responsive element binding protein/brain derived neurotrophic factor signaling pathways in vivo, as well as promote the expression of postsynaptic density (PSD) proteins, PSD95 and Homer1. PMID:31820273
  31. At the molecular level, FG 4592 was found to activate HIF 1 and cAMP responsive element binding protein/brain derived neurotrophic factor signaling pathways in vivo, as well as promote the expression of postsynaptic density (PSD) proteins, PSD95 and Homer1. PMID:31820273
  32. At the molecular level, FG 4592 was found to activate HIF 1 and cAMP responsive element binding protein/brain derived neurotrophic factor signaling pathways in vivo, as well as promote the expression of postsynaptic density (PSD) proteins, PSD95 and Homer1. PMID:31820273
  33. We utilized outbred wild type (WT) and rats selectively bred for low voluntary running (LVR) behavior to address whether mPOA ERβ signaling blunts ERα driven wheel running behavior and immediate early gene (Fos, Zif268, and Homer1) mRNA induction. PMID:31711895
  34. Similarly, ERα agonism (PPT) increased Fos and Homer1 induction ∼3 fold in WT and LVR isolated mPOA neurons, with no effect of the ERβ agonist DPN alone or in combination with PPT, suggesting medial preoptic ERβ activity may blunt ERα signaling. PMID:31711895
  35. Consistent with this in vivo observation, we found that lack of Rhes in mice caused a greater increase in striatal cocaine dependent D1R/cAMP/PKA signaling, along with considerable enhancement of Arc, zif268, and Homer1 mRNA expression. PMID:31653935
  36. Consistent with this in vivo observation, we found that lack of Rhes in mice caused a greater increase in striatal cocaine dependent D1R/cAMP/PKA signaling, along with considerable enhancement of Arc, zif268, and Homer1 mRNA expression. PMID:31653935
  37. The CaSR Homer1 protein complex, whose formation was increased in response to extracellular Ca2+, was bound to mechanistic target of rapamycin (mTOR) complex 2 (mTORC2), a protein kinase that phosphorylates and activates AKT Ser/Thr kinase (AKT) at Ser473 siRNA based gene silencing assays with primary osteoblasts revealed that both CaSR and Homer1 are required for extracellular Ca2+ stimulated AKT phosphorylation and thereby inhibit apoptosis and promote AKT dependent β catenin stabilization and cellular differentiation. PMID:31527082
  38. The CaSR Homer1 protein complex, whose formation was increased in response to extracellular Ca2+, was bound to mechanistic target of rapamycin (mTOR) complex 2 (mTORC2), a protein kinase that phosphorylates and activates AKT Ser/Thr kinase (AKT) at Ser473 siRNA based gene silencing assays with primary osteoblasts revealed that both CaSR and Homer1 are required for extracellular Ca2+ stimulated AKT phosphorylation and thereby inhibit apoptosis and promote AKT dependent β catenin stabilization and cellular differentiation. PMID:31527082
  39. To confirm the role of the CaSR Homer1 complex in AKT initiation, we show that in HEK 293 cells, co transfection with both Homer1c and CaSR, but neither with Homer1c nor CaSR alone, establishes sensitivity of AKT Ser473 phosphorylation to increases in extracellular Ca2+ concentrations. PMID:31527082
  40. Additionally, there was a significant negative correlation between the levels of Homer1 mGluR5 signaling and depressive like behaviors. PMID:31265863
  41. Here, we examined the interaction between Homer1 EVH1 domain and RyR1 by co immunoprecipitation, continuous sucrose density gradient centrifugation, and bio layer interferometry binding assay at different Ca2+ concentrations. PMID:31078268
  42. Our results show that there exists a high affinity binding between the Homer1 EVH1 domain and RyR1, especially at 1 mM of Ca2+. PMID:31078268
  43. Based on our data and the known structures of Homer1 EVH1 domain and RyR1, we found two consensus proline rich sequences in the structure of RyR1, PPHHF and FLPPP, and proposed two corresponding binding models to show mechanisms of recognition different from those used by other proline rich motifs. PMID:31078268
  44. Genes related with cytokine production (il12rb2, scfr), immune system (ig8l, hlfl, cd226, prf1l), autophagy regulation (atg4b), foxo signaling (ccnb2), steroid hormone biosynthesis (cyp3a40), fatty acid metabolism (scd1), metabolism (cacna1b) have been significantly decreased, while genes associated with such pathways above (leap 2, prdx4, mb, homer1, mif, sat1, cytbc1_8) and the pathway of protein processing in endoplasmic reticulum (cne1, tram1) have been significantly increased. PMID:31028934
  45. The same phenotype was recapitulated in a mouse model that has reduced Homer1 binding to metabotropic glutamate receptor 5 (mGluR5). PMID:30867257
  46. The G allele of rs325197091 (A > G) may create a new binding site of transcription factor aryl hydrocarbon receptor nuclear translocator (ARNT) and could enhance HOMER1 intronic promoter activity. PMID:30526478
  47. Our study suggests a potential regulatory role of Homer1 in modulating excitatory synaptic spine homeostatic scaling via binding to Drebrin. PMID:30503778
  48. Furthermore, Parkin regulates synaptic AMPA receptor trafficking via its binding and retention of the postsynaptic scaffold Homer1, and all mutants are similarly impaired in this capacity. PMID:30200940
  49. In depth functional studies showed that a frame shift mutation, FRMPD4p.Cys618ValfsX8, results in a disruption of FRMPD4 binding with PSD 95 and HOMER1, and a failure to increase spine density in transfected hippocampal neurons. PMID:29267967
  50. Our survey demonstrates that the induction of a large number of activity regulated genes, including Arc/Arg3.1, Arl5b, Gadd45b, Homer1, Inhba and Zwint, is indeed dependent on ERK phosphorylation. PMID:28349920
  51. The Impact of Small RNA Interference Against Homer1 on Rats with Type 2 Diabetes and ERK Phosphorylation. PMID:27259299
  52. Homer1 expression in human monocytic THP 1 cells was interfered using short hairpin RNA, and its effect on phosphorylation of extracellular signal regulated kinase (ERK) was assessed. PMID:27259299
  53. Homer1 expression in human monocytic THP 1 cells was interfered using short hairpin RNA, and its effect on phosphorylation of extracellular signal regulated kinase (ERK) was assessed. PMID:27259299
  54. Compared with controls, P ERK phosphorylation was significantly decreased in THP 1 cells with interfered Homer1 (P < 0.05). PMID:27259299
  55. Homer1 can promote the progression of T2DM, which may be achieved through affecting ERK phosphorylation. PMID:27259299
  56. Here, we find that the mGlu5 mediated Ca2+ signaling leading to release of glutamate is governed by mGlu5 interaction with Homer1 scaffolding proteins. PMID:27075036
  57. Furthermore, detailed submodule analyses of important candidate genes (HOMER1, SPTBN1) by all edges within the yellow module implied significant enrichment of functional connections between bone and cytoskeletal protein binding. PMID:26748680
  58. In support, phosphatase treatment of prenatal cocaine exposed tissues restores whereas PKC mediated phosphorylation of saline treated synaptic membrane attenuates mGluR1 coupling to both Gq/11 and Homer1. PMID:24626340
  59. Our results indicate an involvement of Homer1 mediated signaling in the hippocampus in motivation based learning tasks and encourage further investigations regarding the specific molecular underpinnings of the phenotypes observed in this study. PMID:24465821
  60. Homer1 protein is an important scaffold protein at postsynaptic density and has been demonstrated to play a central role in calcium signaling in the central nervous system. PMID:24036210
  61. Variants of Homer1, a key PSD protein for glutamate signaling, have been associated with schizophrenia symptoms severity and therapeutic response. PMID:23999870
  62. We found that scopolamine caused spatial learning and memory deficits that involved activation of glycogen synthase kinase 3β (GSK 3β) and impairments of dendrite arborization and spine formation/maturation associated with alterations of AMPAR, Homer1, and CREB. PMID:23948897
  63. Together, these data provide novel evidence in support of opposing roles for constitutively expressed Homer1 and Homer2 isoforms in regulating mPFC glutamate transmission in vivo and support the hypothesis that cocaine elicited increases in the relative amount of mPFC Homer2 versus Homer1 signaling produces abnormalities in NAC glutamate transmission that enhance vulnerability to cocaine reward. PMID:23658151
  64. Glutamate signaling is processed by different receptors, including metabotropic glutamate receptors (mGluRs), which in turn interact with the scaffolding protein Homer1 to modulate downstream Ca(2+) signaling. PMID:23447597
  65. Cognitive impairments have often been shown to occur after episodes of stress, however the specific role of mGluR5/Homer1 signaling in the interaction of stress and cognition has not yet been elucidated. PMID:23447597
  66. In summary, we could demonstrate that stress induces a cognitive deficit that is likely mediated by mGluR5/Homer1 signaling in the hippocampus. PMID:23447597
  67. Homer1a, the short form of the Homer1 family of scaffolding proteins, disrupts the mGluR signaling complex and negatively regulates nociceptive plasticity at spinal synapses. PMID:21595930
  68. A DNA chip analysis identified 17 up regulated genes in human PDL cells under the mechanical stress, including HOMER1 (homer homolog 1) and GRIN3A (glutamate receptor ionotropic N methyl d aspartate 3A), which are related to glutamate signaling. PMID:20576613
  69. In cultured rat hippocampal neurons, acute and gradual increases in BDNF elicited transient and sustained activation of TrkB receptor and its downstream signaling, respectively, leading to differential expression of Homer1 and Arc. PMID:20173744
  70. Pharmacological experiments confirmed that the operational state of a calcium calmodulin dependent protein kinase II/calcineurin phosphatase molecular switch was dependent on Homer1 expression. PMID:19650914
  71. These experiments provide evidence that Homer1 is an essential component of the calcium signalling repertoire within motile growth cones, regulating guidance cue induced calcium release and maintaining basal cytosolic calcium. PMID:19650914
  72. Short Homer1 (lacking the coiled coil multimerisation domain) and Homer1b similarly modulated RyR2, indicating an action through ligand binding, not mutimerisation. PMID:19296124
  73. Cocaine activates Homer1 immediate early gene transcription in the mesocorticolimbic circuit: differential regulation by dopamine and glutamate signaling. PMID:18932227
  74. A separate quantitative PCR analysis of genes in the last of these groups confirms the dysregulation of both orphan (Gpr88) and known (DrD1A, Adora2A, Cnr1, Grm5, Gpr6) G protein coupled receptors, scaffolding (PSD95, Homer1) and signaling (Sgk, Cap1) proteins, and neuropeptides (CCK, galanin). PMID:18588537
  75. In many cases the Homers function as a buffer to reduce the intensity of Ca(2+) signaling and create a negative bias that can be reversed by the immediate early gene form of Homer1. PMID:17618683
  76. Using microarray and quantitative real time RT PCR approaches, we identified several genes previously known to be associated with memory processes (Homer1, GABA(B) receptor, early growth response 1, prodynorphin, VGF nerve growth factor inducible) and multiple novel candidate genes possibly involved in cognition (including calcium/calmodulin dependent protein kinase kinase 2, dual specificity phosphatase 5 and 6, glycophorin C) that were altered following scopolamine treatment. PMID:17540011
  77. Ca2+ signaling in microdomains: Homer1 mediates the interaction between RyR2 and Cav1.2 to regulate excitation contraction coupling. PMID:17355963
  78. mGluR1alpha, G alpha q/G alpha 11 subunits of G q protein, inositol 1,4,5 trisphosphate receptor IP3R1, Homer1, protein kinase C PKCgamma, and diacylglycerol lipase DAGLalpha. PMID:17298601
  79. Furthermore, a downregulation of calcium/calmodulin dependent protein kinase type IIA and Homer1 was observed. PMID:17241285
  80. Cytoskeletal proteins that interact with the long C terminal tail, such as homer1 and 4.1G, are known to modulate the mGluR1alpha signaling; however, their effects on the dual signaling remain unknown. PMID:17215138
  81. These results indicate that Homer1 proteins regulate synaptic cycling and Ca2+ signaling of mGlu5 receptors, in response to neuronal activity. PMID:12093163